Genetic diversity of MERS-CoV spike protein gene in Saudi Arabia
Identifieur interne : 000557 ( Main/Exploration ); précédent : 000556; suivant : 000558Genetic diversity of MERS-CoV spike protein gene in Saudi Arabia
Auteurs : Sayed S. Sohrab [Arabie saoudite] ; Esam I. Azhar [Arabie saoudite]Source :
- Journal of Infection and Public Health [ 1876-0341 ] ; 2019.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) was primarily detected in 2012 and still causing disease in human and camel. Camel and bats have been identified as a potential source of virus for disease spread to human. Although, significant information related to MERS-CoV disease, spread, infection, epidemiology, clinical features have been published, A little information is available on the sequence diversity of Spike protein gene. The Spike protein gene plays a significant role in virus attachment to host cells. Recently, the information about recombinant MERS-CoV has been published. So, this work was designed to identify the emergence of any another recombinant virus in Jeddah, Saudi Arabia.
In this study samples were collected from both human and camels and the Spike protein gene was amplified and sequenced. The nucleotide and amino acid sequences of MERS-CoV Spike protein gene were used to analyze the recombination, genetic diversity and phylogenetic relationship with selected sequences from Saudi Arabia.
The nucleotide sequence identity ranged from 65.7% to 99.8% among all the samples collected from human and camels from various locations in the Kingdom. The lowest similarity (65.7%) was observed in samples from Madinah and Dammam. The phylogenetic relationship formed different clusters with multiple isolates from various locations. The sample collected from human in Jeddah hospital formed a closed cluster with human samples collected from Buraydah, while camel sample formed a closed cluster with Hufuf isolates. The phylogenetic tree by using Aminoacid sequences formed closed cluster with Dammam, Makkah and Duba isolates. The amino acid sequences variations were observed in 28/35 samples and two unique amino acid sequences variations were observed in all samples analyzed while total 19 nucleotides sequences variations were observed in the Spike protein gene. The minor recombination events were identified in eight different sequences at various hotspots in both human and camel samples using recombination detection programme.
The generated information from this study is very valuable and it will be used to design and develop therapeutic compounds and vaccine to control the MERS-CoV disease spread in not only in the Kingdom but also globally.
Url:
DOI: 10.1016/j.jiph.2019.11.007
PubMed: 31831395
PubMed Central: 7102590
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000E62
- to stream Pmc, to step Curation: 000E62
- to stream Pmc, to step Checkpoint: 000298
- to stream PubMed, to step Corpus: 000333
- to stream PubMed, to step Curation: 000333
- to stream PubMed, to step Checkpoint: 000545
- to stream Ncbi, to step Merge: 002431
- to stream Ncbi, to step Curation: 002431
- to stream Ncbi, to step Checkpoint: 002431
- to stream Main, to step Merge: 000560
- to stream Main, to step Curation: 000557
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genetic diversity of MERS-CoV spike protein gene in Saudi Arabia</title><author><name sortKey="Sohrab, Sayed S" sort="Sohrab, Sayed S" uniqKey="Sohrab S" first="Sayed S." last="Sohrab">Sayed S. Sohrab</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589</wicri:regionArea><wicri:noRegion>21589</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589</wicri:regionArea><wicri:noRegion>21589</wicri:noRegion></affiliation></author><author><name sortKey="Azhar, Esam I" sort="Azhar, Esam I" uniqKey="Azhar E" first="Esam I." last="Azhar">Esam I. Azhar</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589</wicri:regionArea><wicri:noRegion>21589</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589</wicri:regionArea><wicri:noRegion>21589</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31831395</idno><idno type="pmc">7102590</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102590</idno><idno type="RBID">PMC:7102590</idno><idno type="doi">10.1016/j.jiph.2019.11.007</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000E62</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E62</idno><idno type="wicri:Area/Pmc/Curation">000E62</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E62</idno><idno type="wicri:Area/Pmc/Checkpoint">000298</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000298</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:31831395</idno><idno type="wicri:Area/PubMed/Corpus">000333</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000333</idno><idno type="wicri:Area/PubMed/Curation">000333</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000333</idno><idno type="wicri:Area/PubMed/Checkpoint">000545</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000545</idno><idno type="wicri:Area/Ncbi/Merge">002431</idno><idno type="wicri:Area/Ncbi/Curation">002431</idno><idno type="wicri:Area/Ncbi/Checkpoint">002431</idno><idno type="wicri:doubleKey">1876-0341:2019:Sohrab S:genetic:diversity:of</idno><idno type="wicri:Area/Main/Merge">000560</idno><idno type="wicri:Area/Main/Curation">000557</idno><idno type="wicri:Area/Main/Exploration">000557</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genetic diversity of MERS-CoV spike protein gene in Saudi Arabia</title><author><name sortKey="Sohrab, Sayed S" sort="Sohrab, Sayed S" uniqKey="Sohrab S" first="Sayed S." last="Sohrab">Sayed S. Sohrab</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589</wicri:regionArea><wicri:noRegion>21589</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589</wicri:regionArea><wicri:noRegion>21589</wicri:noRegion></affiliation></author><author><name sortKey="Azhar, Esam I" sort="Azhar, Esam I" uniqKey="Azhar E" first="Esam I." last="Azhar">Esam I. Azhar</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589</wicri:regionArea><wicri:noRegion>21589</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589</wicri:regionArea><wicri:noRegion>21589</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Infection and Public Health</title><idno type="ISSN">1876-0341</idno><idno type="eISSN">1876-035X</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Middle East respiratory syndrome coronavirus (MERS-CoV) was primarily detected in 2012 and still causing disease in human and camel. Camel and bats have been identified as a potential source of virus for disease spread to human. Although, significant information related to MERS-CoV disease, spread, infection, epidemiology, clinical features have been published, A little information is available on the sequence diversity of Spike protein gene. The Spike protein gene plays a significant role in virus attachment to host cells. Recently, the information about recombinant MERS-CoV has been published. So, this work was designed to identify the emergence of any another recombinant virus in Jeddah, Saudi Arabia.</p></sec><sec><title>Methods</title><p>In this study samples were collected from both human and camels and the Spike protein gene was amplified and sequenced. The nucleotide and amino acid sequences of MERS-CoV Spike protein gene were used to analyze the recombination, genetic diversity and phylogenetic relationship with selected sequences from Saudi Arabia.</p></sec><sec><title>Results</title><p>The nucleotide sequence identity ranged from 65.7% to 99.8% among all the samples collected from human and camels from various locations in the Kingdom. The lowest similarity (65.7%) was observed in samples from Madinah and Dammam. The phylogenetic relationship formed different clusters with multiple isolates from various locations. The sample collected from human in Jeddah hospital formed a closed cluster with human samples collected from Buraydah, while camel sample formed a closed cluster with Hufuf isolates. The phylogenetic tree by using Aminoacid sequences formed closed cluster with Dammam, Makkah and Duba isolates. The amino acid sequences variations were observed in 28/35 samples and two unique amino acid sequences variations were observed in all samples analyzed while total 19 nucleotides sequences variations were observed in the Spike protein gene. The minor recombination events were identified in eight different sequences at various hotspots in both human and camel samples using recombination detection programme.</p></sec><sec><title>Conclusion</title><p>The generated information from this study is very valuable and it will be used to design and develop therapeutic compounds and vaccine to control the MERS-CoV disease spread in not only in the Kingdom but also globally.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Brian, D A" uniqKey="Brian D">D.A. Brian</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weiss, S R" uniqKey="Weiss S">S.R. Weiss</name></author><author><name sortKey="Navas Martin, S" uniqKey="Navas Martin S">S. Navas-Martin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Su, S" uniqKey="Su S">S. Su</name></author><author><name sortKey="Wong, G" uniqKey="Wong G">G. Wong</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W. Shi</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J. Liu</name></author><author><name sortKey="Lai, A C K" uniqKey="Lai A">A.C.K. Lai</name></author><author><name sortKey="Zhou, J" uniqKey="Zhou J">J. Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author><author><name sortKey="Mishra, N" uniqKey="Mishra N">N. Mishra</name></author><author><name sortKey="Olival, K J" uniqKey="Olival K">K.J. Olival</name></author><author><name sortKey="Fagbo, S F" uniqKey="Fagbo S">S.F. Fagbo</name></author><author><name sortKey="Kapoor, V" uniqKey="Kapoor V">V. Kapoor</name></author><author><name sortKey="Epstein, J H" uniqKey="Epstein J">J.H. Epstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Azhar, E I" uniqKey="Azhar E">E.I. Azhar</name></author><author><name sortKey="El Kafrawy, S A" uniqKey="El Kafrawy S">S.A. El-Kafrawy</name></author><author><name sortKey="Farraj, S A" uniqKey="Farraj S">S.A. Farraj</name></author><author><name sortKey="Hassan, A M" uniqKey="Hassan A">A.M. Hassan</name></author><author><name sortKey="Al Saeed, M S" uniqKey="Al Saeed M">M.S. Al-Saeed</name></author><author><name sortKey="Hashem, A M" uniqKey="Hashem A">A.M. Hashem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oboho, I K" uniqKey="Oboho I">I.K. Oboho</name></author><author><name sortKey="Tomczyk, S M" uniqKey="Tomczyk S">S.M. Tomczyk</name></author><author><name sortKey="Al Asmari, A M" uniqKey="Al Asmari A">A.M. Al-Asmari</name></author><author><name sortKey="Banjar, A A" uniqKey="Banjar A">A.A. Banjar</name></author><author><name sortKey="Al Mugti, H" uniqKey="Al Mugti H">H. Al-Mugti</name></author><author><name sortKey="Aloraini, M S" uniqKey="Aloraini M">M.S. Aloraini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Assiri, A" uniqKey="Assiri A">A. Assiri</name></author><author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. Al-Tawfiq</name></author><author><name sortKey="Al Rabeeah, A A" uniqKey="Al Rabeeah A">A.A. Al-Rabeeah</name></author><author><name sortKey="Al Rabiah, F A" uniqKey="Al Rabiah F">F.A. Al-Rabiah</name></author><author><name sortKey="Al Hajjar, S" uniqKey="Al Hajjar S">S. Al-Hajjar</name></author><author><name sortKey="Al Barrak, A" uniqKey="Al Barrak A">A. Al-Barrak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Seilmaier, M" uniqKey="Seilmaier M">M. Seilmaier</name></author><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Hartmann, W" uniqKey="Hartmann W">W. Hartmann</name></author><author><name sortKey="Scheible, G" uniqKey="Scheible G">G. Scheible</name></author><author><name sortKey="Sack, S" uniqKey="Sack S">S. Sack</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Müller</name></author><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Al Masri, M" uniqKey="Al Masri M">M. Al-Masri</name></author><author><name sortKey="Hossain, R" uniqKey="Hossain R">R. Hossain</name></author><author><name sortKey="Madani, H" uniqKey="Madani H">H. Madani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, S S" uniqKey="Lee S">S.S. Lee</name></author><author><name sortKey="Wong, N S" uniqKey="Wong N">N.S. Wong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Azhar, E I" uniqKey="Azhar E">E.I. Azhar</name></author><author><name sortKey="Hashem, A M" uniqKey="Hashem A">A.M. Hashem</name></author><author><name sortKey="El Kafrawy, S A" uniqKey="El Kafrawy S">S.A. El-Kafrawy</name></author><author><name sortKey="Sohrab, S S" uniqKey="Sohrab S">S.S. Sohrab</name></author><author><name sortKey="Aburizaiza, A S" uniqKey="Aburizaiza A">A.S. Aburizaiza</name></author><author><name sortKey="Farraj, S A" uniqKey="Farraj S">S.A. Farraj</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y. Chen</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Kang, Y" uniqKey="Kang Y">Y. Kang</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name></author><author><name sortKey="Luk, H K" uniqKey="Luk H">H.K. Luk</name></author><author><name sortKey="Poon, R W" uniqKey="Poon R">R.W. Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ali, M" uniqKey="Ali M">M. Ali</name></author><author><name sortKey="El Shesheny, R" uniqKey="El Shesheny R">R. El-Shesheny</name></author><author><name sortKey="Kandeil, A" uniqKey="Kandeil A">A. Kandeil</name></author><author><name sortKey="Shehata, M" uniqKey="Shehata M">M. Shehata</name></author><author><name sortKey="Elsokary, B" uniqKey="Elsokary B">B. Elsokary</name></author><author><name sortKey="Gomaa, M" uniqKey="Gomaa M">M. Gomaa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, D W" uniqKey="Kim D">D.W. Kim</name></author><author><name sortKey="Kim, Y J" uniqKey="Kim Y">Y.J. Kim</name></author><author><name sortKey="Park, S H" uniqKey="Park S">S.H. Park</name></author><author><name sortKey="Yun, M R" uniqKey="Yun M">M.R. Yun</name></author><author><name sortKey="Yang, J S" uniqKey="Yang J">J.S. Yang</name></author><author><name sortKey="Kang, H J" uniqKey="Kang H">H.J. Kang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Y" uniqKey="Kim Y">Y. Kim</name></author><author><name sortKey="Cheon, S" uniqKey="Cheon S">S. Cheon</name></author><author><name sortKey="Min, C K" uniqKey="Min C">C.K. Min</name></author><author><name sortKey="Sohn, K M" uniqKey="Sohn K">K.M. Sohn</name></author><author><name sortKey="Kang, Y J" uniqKey="Kang Y">Y.J. Kang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ali, S" uniqKey="Ali S">S. Ali</name></author><author><name sortKey="Omrani, Jaffar A" uniqKey="Omrani J">Jaffar A. Omrani</name></author><author><name sortKey="Al Tawfiq" uniqKey="Al Tawfiq">Al-Tawfiq</name></author><author><name sortKey="Memish, Ziad A" uniqKey="Memish Z">Ziad A. Memish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Amer, H" uniqKey="Amer H">H. Amer</name></author><author><name sortKey="Alqahtani, A S" uniqKey="Alqahtani A">A.S. Alqahtani</name></author><author><name sortKey="Alzoman, H" uniqKey="Alzoman H">H. Alzoman</name></author><author><name sortKey="Aljerian, N" uniqKey="Aljerian N">N. Aljerian</name></author><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yusof, M F" uniqKey="Yusof M">M.F. Yusof</name></author><author><name sortKey="Queen, K" uniqKey="Queen K">K. Queen</name></author><author><name sortKey="Eltahir, Y M" uniqKey="Eltahir Y">Y.M. Eltahir</name></author><author><name sortKey="Paden, C R" uniqKey="Paden C">C.R. Paden</name></author><author><name sortKey="Al Hammadi, Z M A H" uniqKey="Al Hammadi Z">Z.M.A.H. Al Hammadi</name></author><author><name sortKey="Tao, Y" uniqKey="Tao Y">Y. Tao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paden, C R" uniqKey="Paden C">C.R. Paden</name></author><author><name sortKey="Yusof, M F B M" uniqKey="Yusof M">M.F.B.M. Yusof</name></author><author><name sortKey="Al Hammadi, Z M" uniqKey="Al Hammadi Z">Z.M. Al Hammadi</name></author><author><name sortKey="Queen, K" uniqKey="Queen K">K. Queen</name></author><author><name sortKey="Tao, Y" uniqKey="Tao Y">Y. Tao</name></author><author><name sortKey="Eltahir, Y M" uniqKey="Eltahir Y">Y.M. Eltahir</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dudas, G" uniqKey="Dudas G">G. Dudas</name></author><author><name sortKey="Carvalho, L M" uniqKey="Carvalho L">L.M. Carvalho</name></author><author><name sortKey="Rambaut, A" uniqKey="Rambaut A">A. Rambaut</name></author><author><name sortKey="Bedford, T" uniqKey="Bedford T">T. Bedford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Millet, J K" uniqKey="Millet J">J.K. Millet</name></author><author><name sortKey="Licitra, B N" uniqKey="Licitra B">B.N. Licitra</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Mou, H" uniqKey="Mou H">H. Mou</name></author><author><name sortKey="Smits, S L" uniqKey="Smits S">S.L. Smits</name></author><author><name sortKey="Dekkers, D H" uniqKey="Dekkers D">D.H. Dekkers</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Müller</name></author><author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R. Dijkman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, N" uniqKey="Wang N">N. Wang</name></author><author><name sortKey="Shi, X" uniqKey="Shi X">X. Shi</name></author><author><name sortKey="Jiang, L" uniqKey="Jiang L">L. Jiang</name></author><author><name sortKey="Zhang, S" uniqKey="Zhang S">S. Zhang</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author><author><name sortKey="Tong, P" uniqKey="Tong P">P. Tong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Coleman, C M" uniqKey="Coleman C">C.M. Coleman</name></author><author><name sortKey="Venkataraman, T" uniqKey="Venkataraman T">T. Venkataraman</name></author><author><name sortKey="Liu, Y V" uniqKey="Liu Y">Y.V. Liu</name></author><author><name sortKey="Glenn, G M" uniqKey="Glenn G">G.M. Glenn</name></author><author><name sortKey="Smith, G E" uniqKey="Smith G">G.E. Smith</name></author><author><name sortKey="Flyer, D C" uniqKey="Flyer D">D.C. Flyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, D W" uniqKey="Kim D">D.W. Kim</name></author><author><name sortKey="Kim, Y J" uniqKey="Kim Y">Y.J. Kim</name></author><author><name sortKey="Park, S H" uniqKey="Park S">S.H. Park</name></author><author><name sortKey="Yun, M R" uniqKey="Yun M">M.R. Yun</name></author><author><name sortKey="Yang, J S" uniqKey="Yang J">J.S. Yang</name></author><author><name sortKey="Kang, H J" uniqKey="Kang H">H.J. Kang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Assiri, A M" uniqKey="Assiri A">A.M. Assiri</name></author><author><name sortKey="Midgley, C M" uniqKey="Midgley C">C.M. Midgley</name></author><author><name sortKey="Abedi, G R" uniqKey="Abedi G">G.R. Abedi</name></author><author><name sortKey="Bin Saeed, A" uniqKey="Bin Saeed A">A. Bin Saeed</name></author><author><name sortKey="Almasri, M M" uniqKey="Almasri M">M.M. Almasri</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X. Lu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiaoyan, Lu" uniqKey="Xiaoyan L">Lu Xiaoyan</name></author><author><name sortKey="Lori, A" uniqKey="Lori A">A. Lori</name></author><author><name sortKey="Rowe, M F" uniqKey="Rowe M">M.F. Rowe</name></author><author><name sortKey="James, S" uniqKey="James S">S. James</name></author><author><name sortKey="Glen, R" uniqKey="Glen R">R. Glen</name></author><author><name sortKey="Abedi, O E" uniqKey="Abedi O">O.E. Abedi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anthony, S J" uniqKey="Anthony S">S.J. Anthony</name></author><author><name sortKey="Gilardi, K" uniqKey="Gilardi K">K. Gilardi</name></author><author><name sortKey="Menachery, V D" uniqKey="Menachery V">V.D. Menachery</name></author><author><name sortKey="Goldstein, T" uniqKey="Goldstein T">T. Goldstein</name></author><author><name sortKey="Ssebide, B" uniqKey="Ssebide B">B. Ssebide</name></author><author><name sortKey="Mbabazi" uniqKey="Mbabazi">Mbabazi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chu, D K W" uniqKey="Chu D">D.K.W. Chu</name></author><author><name sortKey="Hui, K P Y" uniqKey="Hui K">K.P.Y. Hui</name></author><author><name sortKey="Perera, R A P M" uniqKey="Perera R">R.A.P.M. Perera</name></author><author><name sortKey="Miguel, E" uniqKey="Miguel E">E. Miguel</name></author><author><name sortKey="Niemeyer, D" uniqKey="Niemeyer D">D. Niemeyer</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chung, Y S" uniqKey="Chung Y">Y.S. Chung</name></author><author><name sortKey="Kim, J M" uniqKey="Kim J">J.M. Kim</name></author><author><name sortKey="Man Kim, H" uniqKey="Man Kim H">H. Man Kim</name></author><author><name sortKey="Park, K R" uniqKey="Park K">K.R. Park</name></author><author><name sortKey="Lee, A" uniqKey="Lee A">A. Lee</name></author><author><name sortKey="Lee, N J" uniqKey="Lee N">N.J. Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kumar, S" uniqKey="Kumar S">S. Kumar</name></author><author><name sortKey="Stecher, G" uniqKey="Stecher G">G. Stecher</name></author><author><name sortKey="Tamura, K" uniqKey="Tamura K">K. Tamura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martin, D P" uniqKey="Martin D">D.P. Martin</name></author><author><name sortKey="Murrell, B" uniqKey="Murrell B">B. Murrell</name></author><author><name sortKey="Golden, M" uniqKey="Golden M">M. Golden</name></author><author><name sortKey="Khoosal, A" uniqKey="Khoosal A">A. Khoosal</name></author><author><name sortKey="Muhire, B" uniqKey="Muhire B">B. Muhire</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Borucki, M K" uniqKey="Borucki M">M.K. Borucki</name></author><author><name sortKey="Lao, V" uniqKey="Lao V">V. Lao</name></author><author><name sortKey="Hwang, M" uniqKey="Hwang M">M. Hwang</name></author><author><name sortKey="Gardner, S" uniqKey="Gardner S">S. Gardner</name></author><author><name sortKey="Adney, D" uniqKey="Adney D">D. Adney</name></author><author><name sortKey="Munster, V" uniqKey="Munster V">V. Munster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K. Pyrc</name></author><author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R. Dijkman</name></author><author><name sortKey="Deng, L" uniqKey="Deng L">L. Deng</name></author><author><name sortKey="Jebbink, M F" uniqKey="Jebbink M">M.F. Jebbink</name></author><author><name sortKey="Ross, H A" uniqKey="Ross H">H.A. Ross</name></author><author><name sortKey="Berkhout, B" uniqKey="Berkhout B">B. Berkhout</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cotten, M" uniqKey="Cotten M">M. Cotten</name></author><author><name sortKey="Watson, S J" uniqKey="Watson S">S.J. Watson</name></author><author><name sortKey="Zumla, A I" uniqKey="Zumla A">A.I. Zumla</name></author><author><name sortKey="Makhdoom, H Q" uniqKey="Makhdoom H">H.Q. Makhdoom</name></author><author><name sortKey="Palser, A L" uniqKey="Palser A">A.L. Palser</name></author><author><name sortKey="Ong, S H" uniqKey="Ong S">S.H. Ong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sabir, J S" uniqKey="Sabir J">J.S. Sabir</name></author><author><name sortKey="Lam, T T" uniqKey="Lam T">T.T. Lam</name></author><author><name sortKey="Ahmed, M M" uniqKey="Ahmed M">M.M. Ahmed</name></author><author><name sortKey="Li, L" uniqKey="Li L">L. Li</name></author><author><name sortKey="Shen, Y" uniqKey="Shen Y">Y. Shen</name></author><author><name sortKey="Abo Aba, S E" uniqKey="Abo Aba S">S.E. Abo-Aba</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name></author><author><name sortKey="Lau, S K" uniqKey="Lau S">S.K. Lau</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y. Huang</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, G" uniqKey="Lu G">G. Lu</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Gao, G F" uniqKey="Gao G">G.F. Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gorry, P R" uniqKey="Gorry P">P.R. Gorry</name></author><author><name sortKey="Ahmad, F" uniqKey="Ahmad F">F. Ahmad</name></author><author><name sortKey="Mohl, J" uniqKey="Mohl J">J. Mohl</name></author><author><name sortKey="Alkhatib, G" uniqKey="Alkhatib G">G. Alkhatib</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ahmad, Fahim" uniqKey="Ahmad F">Fahim Ahmad</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ahmad, F" uniqKey="Ahmad F">F. Ahmad</name></author><author><name sortKey="Siddiqui, A" uniqKey="Siddiqui A">A. Siddiqui</name></author><author><name sortKey="Kamal, M A" uniqKey="Kamal M">M.A. Kamal</name></author><author><name sortKey="Sohrab, S S" uniqKey="Sohrab S">S.S. Sohrab</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ahmad, F" uniqKey="Ahmad F">F. Ahmad</name></author><author><name sortKey="Siddiqui, A" uniqKey="Siddiqui A">A. Siddiqui</name></author><author><name sortKey="Raut, A A" uniqKey="Raut A">A.A. Raut</name></author><author><name sortKey="Pandey, P K" uniqKey="Pandey P">P.K. Pandey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Borker, M" uniqKey="Borker M">M. Borker</name></author><author><name sortKey="Ahmad, F" uniqKey="Ahmad F">F. Ahmad</name></author><author><name sortKey="Khan, F" uniqKey="Khan F">F. Khan</name></author><author><name sortKey="Agrawal, S" uniqKey="Agrawal S">S. Agrawal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Müller</name></author><author><name sortKey="Costabel, U" uniqKey="Costabel U">U. Costabel</name></author><author><name sortKey="Timm, J" uniqKey="Timm J">J. Timm</name></author><author><name sortKey="Binger, T" uniqKey="Binger T">T. Binger</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Arabie saoudite</li></country></list><tree><country name="Arabie saoudite"><noRegion><name sortKey="Sohrab, Sayed S" sort="Sohrab, Sayed S" uniqKey="Sohrab S" first="Sayed S." last="Sohrab">Sayed S. Sohrab</name></noRegion><name sortKey="Azhar, Esam I" sort="Azhar, Esam I" uniqKey="Azhar E" first="Esam I." last="Azhar">Esam I. Azhar</name><name sortKey="Azhar, Esam I" sort="Azhar, Esam I" uniqKey="Azhar E" first="Esam I." last="Azhar">Esam I. Azhar</name><name sortKey="Sohrab, Sayed S" sort="Sohrab, Sayed S" uniqKey="Sohrab S" first="Sayed S." last="Sohrab">Sayed S. Sohrab</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000557 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000557 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:7102590
|texte= Genetic diversity of MERS-CoV spike protein gene in Saudi Arabia
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31831395" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |